NUP85 Polyclonal antibody

Name: NUP85 Polyclonal antibody
Brand: Proteintech
SKU: 19370-1-AP

NUP85 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19370-1-AP

Synonyms

85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

Validation Data Gallery

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AllWBIHCIF

WB analysis of mouse lung using 19370-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 19370-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19370-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19370-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 19370-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 19370-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 19370-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 19370-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE1 cells using 19370-1-AP
IF result (Nucleus stain) of anti-NUP85 (19370-1-AP; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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Cat No. 19370-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse lung tissue, HeLa cells, human brain tissue, mouse thymus tissue
Positive IHC detected in	human stomach tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 2 publications below

Product Information

19370-1-AP targets NUP85 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUP85 fusion protein Ag7086 相同性解析による交差性が予測される生物種
Full Name	nucleoporin 85kDa
Calculated molecular weight	75 kDa
Observed molecular weight	70-75 kDa
GenBank accession number	BC000697
Gene symbol	NUP85
Gene ID (NCBI)	79902
RRID	AB_10859826
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NUP85 antibody 19370-1-AP	Download protocol
IHC protocol for NUP85 antibody 19370-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. Authors - Bin Chen View Article
human	WB	Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. Authors - Ruona Shi View Article
human	WB	Hum Mol Genet Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders. Authors - Ethiraj Ravindran View Article
human	WB,IF	Front Microbiol Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein Authors - Yue-Huan Ling KD Validated View Article
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human	WB	Front Neurol Case report: Compound heterozygous NUP85 variants cause autosomal recessive primary microcephaly Authors - Ethiraj Ravindran View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP85 Polyclonal antibody

NUP85 Polyclonal Antibody for WB, IF, IHC, ELISA

Validation Data Gallery

WB analysis of mouse lung using 19370-1-AP

WB analysis of mouse thymus using 19370-1-AP

WB analysis of human brain using 19370-1-AP

WB analysis of HeLa using 19370-1-AP

IHC staining of human stomach using 19370-1-AP

IHC staining of human stomach using 19370-1-AP

IHC staining of human liver using 19370-1-AP

IHC staining of human liver using 19370-1-AP

IF Staining of hTERT-RPE1 cells using 19370-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.

Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders.

Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein

The mechanism of acentrosomal spindle assembly in human oocytes

Case report: Compound heterozygous NUP85 variants cause autosomal recessive primary microcephaly