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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP85 Polyclonal antibody, PBS Only

NUP85 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30158-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP85 Polyclonal antibody (30158-1-AP)

    WB analysis using 30158-1-AP (same clone as 30158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (Pericentrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUP85 Polyclonal antibody (30158-1-AP)

    WB analysis using 30158-1-AP (same clone as 30158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30158-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30158-1-PBS targets NUP85 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP85 fusion protein Ag33004 相同性解析による交差性が予測される生物種
Full Name nucleoporin 85kDa
Calculated molecular weight 75 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000697
Gene symbol NUP85
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.