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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP85 Recombinant antibody

NUP85 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240116E10

Cat no : 83288-2-RR

Synonyms

Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP85 Recombinant antibody (83288-2-RR)

    WB analysis using 83288-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using NUP85 Recombinant antibody (83288-2-RR)

    FC experiment of MCF-7 using 83288-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83288-2-RR

    Affinity and Kinetic Characterization of 83288-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83288-2-RR against Human NUP85 were performed. The affinity constant is 27.3 nM.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, HepG2 cells, COLO 320 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83288-2-RR is NUP85 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUP85 fusion protein Ag7086 相同性解析による交差性が予測される生物種
Full Name nucleoporin 85kDa
Calculated molecular weight 75 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000697
Gene symbol NUP85
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.

Protocols

Product Specific Protocols
WB protocol for NUP85 antibody 83288-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols