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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP85 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.313-40 ng/mL

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00330-1

Print datasheet

Synonyms

85 kDa nucleoporin, FLJ12549, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85, PCNT1, Pericentrin 1

Validation Data Gallery

  • Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

    Cytometric bead array standard curve of MP00330-1

    Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

  • Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

    Sandwich ELISA standard curve of MP00330-1

    Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

  • The mean NUP85 concentration was determined to be 1,874.5 pg/mL in mouse liver cell extract based on a 3.0 mg/mL extract load.

    Sample test of MP00330-1

    The mean NUP85 concentration was determined to be 1,874.5 pg/mL in mouse liver cell extract based on a 3.0 mg/mL extract load.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00330-1 targets NUP85 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.313-40 ng/mL (Cytometric Bead Array); 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: NUP85 Recombinant antibody, PBS Only (Capture) 83288-2-PBS (240116E10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: NUP85 Recombinant antibody, PBS Only (Detector) 83288-1-PBS (240116A2). 100 μg. Concentration 1 mgl/ml.

Alternative NUP85 matched antibody pairs: MP00330-2

Matched pair protein standard: Ag7086

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83288-2-PBS
Tested Reactivity human
Immunogen NUP85 fusion protein Ag7086
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleoporin 85kDa
Synonyms Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin
GenBank accession numberBC000697
Gene symbol NUP85
UniprotQ9BW27
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83288-1-PBS
Tested Reactivity human
Immunogen NUP85 fusion protein Ag7086
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleoporin 85kDa
Synonyms Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin
GenBank accession numberBC000697
Gene symbol NUP85
UniprotQ9BW27
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.