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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of HEK-293 using 16587-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of human liver using 16587-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of L02 using 16587-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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NUPL2 Polyclonal antibody

NUPL2 Polyclonal Antibody for WB, ELISA
Cat No. 16587-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CG1, hCG1, NLP 1, NLP_1, NLP1, Nucleoporin hCG1, nucleoporin like 2, Nucleoporin like protein 2, NUP42 homolog, NUPL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16587-1-AP targets NUPL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUPL2 fusion protein Ag8816 相同性解析による交差性が予測される生物種
Full Name nucleoporin like 2
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa, 33-36 kDa
GenBank accession numberBC005327
Gene Symbol NUPL2
Gene ID (NCBI) 11097
RRIDAB_2267702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15504
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUPL2 (also known as NLP-1, or hCG1) is an ubiquitous nuclear protein containing many phenylalanine-glycine (FG) repeats. As the functional orthologue of the budding yeast Nup42/Rip1 nucleoporin, NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm (PMID: 10610322). In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope (PMID: 16000379). NUPL2 localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes CRM1-dependent nuclear protein export (PMID: 22250199).

Protocols

Product Specific Protocols
WB protocol for NUPL2 antibody 16587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Loss of FAM111B protease mutated in hereditary fibrosing poikiloderma negatively regulates telomere length

Authors - Maciej Kliszczak
View Article