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- KD/KO Validated
NUPR1 Polyclonal antibody
NUPR1 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15056-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human colon cancer tissue, human prostate cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 13 publications below |
| IHC | See 8 publications below |
| IF | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
15056-1-AP targets NUPR1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUPR1 fusion protein Ag7039 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear protein 1 |
| Calculated molecular weight | 8 kDa |
| GenBank accession number | BC002434 |
| Gene symbol | NUPR1 |
| Gene ID (NCBI) | 26471 |
| RRID | AB_2878104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NUPR1 antibody 15056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma
| ||
J Cell Physiol Mechanistic study of NUPR1 in bladder cancer development through transcriptional regulation of CCR2 | ||
J Inorg Biochem Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression
| ||
Discov Oncol ATF4/NUPR1 axis promotes cancer cell survival and mediates immunosuppression in clear cell renal cell carcinoma | ||
Pathol Res Pract NUPR1 induces autophagy and promotes the progression of Esophageal squamous cell carcinoma via the MAPK-mTOR pathway | ||
PLoS One Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells. |
