Validation Data Gallery
Tested Applications
| Positive WB detected in | NCI-H1299 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83315-2-RR targets NUPR1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NUPR1 fusion protein Ag7039 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear protein 1 |
| Calculated molecular weight | 8 kDa |
| Observed molecular weight | 10 kDa |
| GenBank accession number | BC002434 |
| Gene Symbol | NUPR1 |
| Gene ID (NCBI) | 26471 |
| RRID | AB_3670981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60356 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa, it also has another isoform of 10 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUPR1 antibody 83315-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |