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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUSAP1 Polyclonal antibody (12024-1-AP)

    WB analysis using 12024-1-AP (same clone as 12024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12024-1-AP (NUSAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUSAP1 Polyclonal antibody (12024-1-AP)

    IP experiment of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    IP result of anti-NUSAP1 (IP:12024-1-AP, 4ug; Detection:12024-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUSAP1 Polyclonal antibody (12024-1-AP)

    IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUSAP1 Polyclonal antibody (12024-1-AP)

    IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NUSAP1 Polyclonal antibody (12024-1-AP)

    IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NUSAP1 Polyclonal antibody (12024-1-AP)

    IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUSAP1 Polyclonal antibody (12024-1-AP)

    IF Staining of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NUSAP1 Polyclonal antibody, PBS Only

NUSAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12024-1-PBS targets NUSAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUSAP1 fusion protein Ag2654 相同性解析による交差性が予測される生物種
Full Name nucleolar and spindle associated protein 1
Calculated molecular weight 440 aa, 49 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC001308
Gene Symbol NUSAP1
Gene ID (NCBI) 51203
RRIDAB_2157789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.