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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NTF2 Polyclonal antibody

NTF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15538-1-AP

Synonyms

NUTF2, Placental protein 15, NUTF 2, Nuclear transport factor 2, NTF-2

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using NTF2 Polyclonal antibody (15538-1-AP)

    WB analysis of K-562 using 15538-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15538-1-AP (NTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NTF2 Polyclonal antibody (15538-1-AP)

    IHC staining of human ovary tumor using 15538-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15538-1-AP (NTF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NTF2 Polyclonal antibody (15538-1-AP)

    IHC staining of human ovary tumor using 15538-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15538-1-AP (NTF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NTF2 Polyclonal antibody (15538-1-AP)

    IF Staining of MCF-7 using 15538-1-AP

    Immunofluorescent analysis of MCF-7 cells using 15538-1-AP (NTF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15538-1-AP targets NTF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NTF2 fusion protein Ag7879 相同性解析による交差性が予測される生物種
Full Name nuclear transport factor 2
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC002348
Gene symbol NTF2
Gene ID (NCBI) 10204
RRIDAB_2157805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NTF2 antibody 15538-1-APDownload protocol
IHC protocol for NTF2 antibody 15538-1-APDownload protocol
IF protocol for NTF2 antibody 15538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols