Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 66063-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66063-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 66063-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66063-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

NTF2 Monoclonal antibody

NTF2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66063-1-Ig

Clone No.4H7F9

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

NTF 2, NTF2, nuclear transport factor 2, NUTF 2, NUTF2, Placental protein 15, PP15

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Hela cells, HepG2 cells, human heart tissue
Positive IHC detected in	human placenta tissue, human breast cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

66063-1-Ig targets NTF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	NTF2 fusion protein Ag7879 相同性解析による交差性が予測される生物種
Full Name	nuclear transport factor 2
Calculated molecular weight	14 kDa
Observed molecular weight	13 kDa
GenBank accession number	BC002348
Gene Symbol	NTF2
Gene ID (NCBI)	10204
RRID	AB_11042770
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P61970
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NTF2 antibody 66063-1-Ig	Download protocol
IHC protocol for NTF2 antibody 66063-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Front Oncol NTF2 Upregulation in HNSCC: a Predictive Marker and Potential Therapeutic Target Associated With Immune Infiltration. Authors - Guangxu Xuan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 66063-1-Ig

WB analysis of HepG2 using 66063-1-Ig

WB analysis of human heart using 66063-1-Ig

IHC staining of human placenta using 66063-1-Ig