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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NVL Monoclonal antibody

NVL Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D8G4

Cat no : 68399-1-Ig

Synonyms

NVL2, Nuclear VCP-like protein, Nuclear VCP like protein, nuclear VCP like, Nuclear valosin-containing protein-like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NVL Monoclonal antibody (68399-1-Ig)

    WB analysis using 68399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Flow cytometry (FC) experiment of HeLa cells using NVL Monoclonal antibody (68399-1-Ig)

    FC experiment of HeLa using 68399-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NVL fusion protein Ag9321 相同性解析による交差性が予測される生物種
Full Name nuclear VCP-like
Calculated molecular weight856aa,95 kDa; 659aa,73 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC012105
Gene symbol NVL
Gene ID (NCBI) 4931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NVL antibody 68399-1-IgDownload protocol
FC protocol for NVL antibody 68399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols