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KD/KO Validated

NXF1 Polyclonal antibody

Name: NXF1 Polyclonal antibody
Brand: Proteintech
SKU: 10328-1-AP

NXF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10328-1-AP

Synonyms

Tip-associated protein, Tip associating protein, Tip associated protein, TAP, mRNA export factor TAP

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis of HeLa using 10328-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10328-1-AP
WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.
WB analysis using 10328-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10328-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10328-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10328-1-AP
IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.
IF Staining of HepG2 using 10328-1-AP
Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10328-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 8 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10328-1-AP targets NXF1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NXF1 fusion protein Ag0413 相同性解析による交差性が予測される生物種
Full Name	nuclear RNA export factor 1
Calculated molecular weight	70 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC004904
Gene symbol	NXF1
Gene ID (NCBI)	10482
RRID	AB_2236500
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

Protocols

Product Specific Protocols
WB protocol for NXF1 antibody 10328-1-AP	Download protocol
IF protocol for NXF1 antibody 10328-1-AP	Download protocol
IP protocol for NXF1 antibody 10328-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Biol Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export. Authors - Zhang Liang L KD Validated View Article
		J Cell Biol Intronless mRNAs transit through nuclear speckles to gain export competence. Authors - Ke Wang View Article
human	WB	Nucleic Acids Res Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy. Authors - Sarah H Stubbs View Article
human	WB	PLoS Genet Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells. Authors - Miao Chen KD Validated View Article
monkey	WB,CoIP	Mol Cell Biol Fragile X mental retardation protein FMRP binds mRNAs in the nucleus. Authors - Kim Miri M KD Validated View Article
human	WB,IF,IP	Chromosoma XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1. Authors - Cohen Hannah R HR View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NXF1 Polyclonal antibody

NXF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10328-1-AP

WB analysis of HeLa using 10328-1-AP

WB analysis using 10328-1-AP

WB analysis of HepG2 using 10328-1-AP

WB analysis of K-562 using 10328-1-AP

IP experiment of HeLa using 10328-1-AP

IF Staining of HepG2 using 10328-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.

Intronless mRNAs transit through nuclear speckles to gain export competence.

Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.

Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.