Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
12185-1-AP targets NXF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NXF2 fusion protein Ag2841 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear RNA export factor 2 |
| Calculated molecular weight | 626 aa, 72 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC015020 |
| Gene Symbol | NXF2 |
| Gene ID (NCBI) | 56001 |
| RRID | AB_2236531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZY0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NXF2, also named as Nuclear RNA export factor 2 or TAPL2, is a 626 amino acid protein, which contains one TAP-C domain, one RRM domain, one NTF2 domain, and four LRR repeats. NXF2 belongs to the NXF family and is expressed almost exclusively in testis.NXF2 localizes in the nucleus and is involved in the export of mRNA from the nucleus to the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NXF2 antibody 12185-1-AP | Download protocol |
| IHC protocol for NXF2 antibody 12185-1-AP | Download protocol |
| IP protocol for NXF2 antibody 12185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem J Nuclear export factor 3 is involved in regulating the expression of TGF-β3 in an mRNA export activity-independent manner in mouse Sertoli cells. |