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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    WB analysis of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NXN Polyclonal antibody (16128-1-AP)

    WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NXN Polyclonal antibody (16128-1-AP)

    WB analysis of human heart using 16128-1-AP (same clone as 16128-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NXN Polyclonal antibody (16128-1-AP)

    IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NXN Polyclonal antibody (16128-1-AP)

    IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NXN Polyclonal antibody, PBS Only

NXN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16128-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16128-1-PBS targets NXN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NXN fusion protein Ag9113 相同性解析による交差性が予測される生物種
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene Symbol NXN
Gene ID (NCBI) 64359
RRIDAB_2158114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DKJ4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.