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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NXN Monoclonal antibody (66603-1-Ig)

    WB analysis using 66603-1-Ig (same clone as 66603-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66603-1-Ig (NXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NXN Monoclonal antibody (66603-1-Ig)

    IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NXN Monoclonal antibody (66603-1-Ig)

    IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NXN Monoclonal antibody (66603-1-Ig)

    IF Staining of HEK-293 using 66603-1-Ig (same clone as 66603-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NXN antibody (66603-1-Ig, Clone: 2C6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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NXN Monoclonal antibody, PBS Only

NXN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66603-1-PBS
Clone No.2C6A4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NRX, nucleoredoxin, NXN, TRG 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66603-1-PBS targets NXN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NXN fusion protein Ag9248 相同性解析による交差性が予測される生物種
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene Symbol NXN
Gene ID (NCBI) 64359
RRIDAB_2881963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6DKJ4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.