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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Napsin A Polyclonal antibody, PBS Only

Napsin A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16558-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using Napsin A Polyclonal antibody (16558-1-AP)

    WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using Napsin A Polyclonal antibody (16558-1-AP)

    WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using Napsin A Polyclonal antibody (16558-1-AP)

    WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human kidney using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human kidney using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Polyclonal antibody (16558-1-AP)

    IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Napsin A Polyclonal antibody (16558-1-AP)

    IF Staining of HUVEC using 16558-1-AP (same clone as 16558-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (16558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16558-1-PBS targets Napsin A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Napsin A fusion protein Ag9786 相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC017842
Gene symbol Napsin A
Gene ID (NCBI) 9476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).