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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Napsin A Monoclonal antibody, PBS Only

Napsin A Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H7F2

Cat no : 60259-2-PBS

Synonyms

NAPSA, KAP, EC:3.4.23.-, Aspartyl protease 4, ASP4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Monoclonal antibody (60259-2-Ig)

    IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Napsin A Monoclonal antibody (60259-2-Ig)

    IF Staining of HUVEC using 60259-2-Ig (same clone as 60259-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Napsin A Monoclonal antibody (60259-2-Ig)

    FC experiment of A549 using 60259-2-Ig (same clone as 60259-2-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60259-2-PBS targets Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Napsin A fusion protein Ag9721 相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
GenBank accession numberBC017842
Gene symbol Napsin A
Gene ID (NCBI) 9476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).