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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using NeuN Monoclonal antibody (66836-1-Ig)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using NeuN Monoclonal antibody (66836-1-Ig)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NeuN Monoclonal antibody (66836-1-Ig)

    IHC staining of human brain using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using NeuN Monoclonal antibody (66836-1-Ig)

    IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NeuN Monoclonal antibody (66836-1-Ig)

    IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using NeuN Monoclonal antibody (66836-1-Ig)

    FC experiment of SH-SY5Y using 66836-1-Ig (same clone as 66836-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NeuN (66836-1-Ig, Clone:3A4C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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NeuN Monoclonal antibody, PBS Only

NeuN Monoclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 66836-1-PBS
Clone No.3A4C1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IHC, IF-P, FC (Intra), ELISA

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66836-1-PBS targets NeuN in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NeuN fusion protein Ag28016 相同性解析による交差性が予測される生物種
Full Name hexaribonucleotide binding protein 3
GenBank accession numberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2882179
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.