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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NeuN Recombinant antibody (84401-4-RR)

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NeuN Recombinant antibody (84401-4-RR)

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of mouse cerebellum using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of mouse cerebellum using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84401-4-RR

    Affinity and Kinetic Characterization of 84401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

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NeuN Recombinant antibody

NeuN Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84401-4-RR
Clone No.241461G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, rat brain tissue
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84401-4-RR targets NeuN in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NeuN fusion protein Ag25689 相同性解析による交差性が予測される生物種
Full Name hexaribonucleotide binding protein 3
Observed molecular weight 46-52 kDa
GenBank accession numberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_3671930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDA6NFN3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NeuN antibody 84401-4-RRDownload protocol
IF protocol for NeuN antibody 84401-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols