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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neudesin/NENF Monoclonal antibody, PBS Only (Capture)

Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G9E12

Cat no : 60131-1-PBS

Synonyms

Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    WB analysis using 60131-1-Ig (same clone as 60131-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    WB analysis of human brain using 60131-1-Ig (same clone as 60131-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IF Staining of HeLa using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Neudesin/NENF Monoclonal antibody (60131-1-Ig)

    IF Staining of HepG2 using 60131-1-Ig (same clone as 60131-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60131-1-Ig(NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50685-1

    Cytometric bead array standard curve of MP50685-1

    Cytometric bead array standard curve of MP50685-1, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-2-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50685-2

    Cytometric bead array standard curve of MP50685-2

    Cytometric bead array standard curve of MP50685-2, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-3-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60131-1-PBS targets Neudesin/NENF as part of a matched antibody pair:

MP50685-1: 60131-1-PBS capture and 60131-2-PBS detection (validated in Cytometric bead array)

MP50685-2: 60131-1-PBS capture and 60131-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Neudesin/NENF fusion protein Ag8387 相同性解析による交差性が予測される生物種
Full Name neuron derived neurotrophic factor
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 19 kDa, 16 kDa
GenBank accession numberBC008823
Gene symbol NENF
Gene ID (NCBI) 29937
RRIDAB_2150987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).