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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neurofibromin 1 Recombinant antibody

Neurofibromin 1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240540F2

Cat no : 83620-5-RR

Synonyms

Neurofibromin, NF1, NF-1, NF 1, 240540F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    WB analysis using 83620-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    FC experiment of U2OS using 83620-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    FC experiment of HeLa using 83620-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA431 cells, PC-12 cell, NIH/3T3 cells, HEK-293T cells, HeLa cells
Positive FC (Intra) detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83620-5-RR is Neurofibromin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Neurofibromin 1 fusion protein Ag25799 相同性解析による交差性が予測される生物種
Full Name neurofibromin 1
Calculated molecular weight 319 kDa
Observed molecular weight250-280 kDa
GenBank accession numberM60915
Gene symbol Neurofibromin 1
Gene ID (NCBI) 4763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

Protocols

Product Specific Protocols
WB protocol for Neurofibromin 1 antibody 83620-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols