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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of HeLa using 60067-1-Ig (same clone as 60067-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of rabbit heart using 60067-1-Ig (same clone as 60067-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of MDA-MB-231 using 60067-1-Ig (same clone as 60067-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of A549 using 60067-1-Ig (same clone as 60067-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of pig heart using 60067-1-Ig (same clone as 60067-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of fetal human brain using 60067-1-Ig (same clone as 60067-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of human plasma using 60067-1-Ig (same clone as 60067-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    WB analysis of pig brain using 60067-1-Ig (same clone as 60067-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human embronic stem cells using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

    Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Neuropilin 1 Monoclonal antibody (60067-1-Ig)

    IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1

    Cytometric bead array standard curve of MP50479-1, NRP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60067-1-PBS. Detection antibody: 60067-2-PBS. Standard:Ag0931. Range: 0.391-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

Neuropilin 1 Monoclonal antibody, PBS Only (Capture)

Neuropilin 1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60067-1-PBS
Clone No.2H3F6

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

NRP1, Neuropilin-1, CD304, BDCA4, 2H3F6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60067-1-PBS targets Neuropilin 1 as part of a matched antibody pair:

MP50479-1: 60067-1-PBS capture and 60067-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuropilin 1 fusion protein Ag0931 相同性解析による交差性が予測される生物種
Full Name neuropilin 1
Calculated molecular weight 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC007737
Gene Symbol Neuropilin 1
Gene ID (NCBI) 8829
RRIDAB_2150840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.