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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neuropilin 1 Recombinant antibody

Neuropilin 1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241812G11

Cat no : 84429-5-RR

Synonyms

NRP1, Neuropilin-1, CD304, BDCA4, 241812G11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neuropilin 1 Recombinant antibody (84429-5-RR)

    WB analysis using 84429-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84429-5-RR (NRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Neuropilin 1 Recombinant antibody (84429-5-RR)

    IHC staining of human placenta using 84429-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Neuropilin 1 Recombinant antibody (84429-5-RR)

    IHC staining of human placenta using 84429-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Neuropilin 1 Recombinant antibody (84429-5-RR)

    IF Staining of PC-3 using 84429-5-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Neuropilin 1 antibody (84429-5-RR, Clone: 241812G11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84429-5-RR targets Neuropilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name neuropilin 1
Observed molecular weight130-140 kDa
GenBank accession numberBC007533
Gene symbol Neuropilin 1
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Protocols

Product Specific Protocols
WB protocol for Neuropilin 1 antibody 84429-5-RRDownload protocol
IHC protocol for Neuropilin 1 antibody 84429-5-RRDownload protocol
IF protocol for Neuropilin 1 antibody 84429-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols