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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neuroserpin Monoclonal antibody, PBS Only

Neuroserpin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4C6

Cat no : 66997-1-PBS

Synonyms

DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using Neuroserpin Monoclonal antibody (66997-1-Ig)

    WB analysis of pig brain using 66997-1-Ig (same clone as 66997-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66997-1-Ig (SERPINI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Neuroserpin Monoclonal antibody (66997-1-Ig)

    IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Neuroserpin Monoclonal antibody (66997-1-Ig)

    IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Neuroserpin Monoclonal antibody (66997-1-Ig)

    IF Staining of U-251 using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Neuroserpin antibody (66997-1-Ig, Clone: 1G4C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66997-1-PBS targets Neuroserpin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuroserpin fusion protein Ag28636 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade I (neuroserpin), member 1
Calculated molecular weight 410 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC018043
Gene symbol SERPINI1
Gene ID (NCBI) 5274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.