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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NeutraKine® EPO Monoclonal antibody

NeutraKine® EPO Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

1D2H2

Cat no : 69019-1-Ig

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2, NeutraKine® EPO

Validation Data Gallery

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  • Neutralization experiment of NeutraKine® EPO using NeutraKine® EPO Monoclonal antibody (69019-1-Ig)

    Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Recombinant human EPO (Cat.NO. HZ-1168) stimulates TF-1 cells (human erythroleukemic cell line) in a dose-dependent nammer (blue curve, refer to bottom X-left Y). The activity of human EPO (6 ng/mL HZ-1168) was neutralized by mouse anti-human EPO monoclonal antibody 69019-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 2-5 μg/mL.

  • Neutralization experiment of NeutraKine® EPO using NeutraKine® EPO Monoclonal antibody (69019-1-Ig)

    Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Recombinant human EPO (Cat.NO. HZ-1168) stimulates TF-1 cells (human erythroleukemic cell line) in a dose-dependent nammer (blue curve, refer to bottom X-left Y). The activity of human EPO (1 ng/mL HZ-1168) was neutralized by mouse anti-human EPO monoclonal antibody 69019-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 2-5 μg/mL. The NeutraControl mouse anti-human EPO monoclonal antibody 69519-1-Ig could recognize human EPO, but could not neutralize human EPO in the range of 0.019-80 μg/mL(green curve, refer to top X-right Y).

  • ELISA experiment of Recombinant protein using NeutraKine® EPO Monoclonal antibody (69019-1-Ig)

    ELISA experiment of Recombinant protein using 69019-1-Ig

    Indirect ELISA was carried out by coating recombinant Human EPO (Cat.NO. HZ-1168) at 70 ng/well followed by blocking and adding serial diluted EPO antibody 69019-1-Ig and 69519-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Neutralization

Product Information

69019-1-Ig targets NeutraKine® EPO in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine EPO protein HZ-1168 相同性解析による交差性が予測される生物種
Full Name erythropoietin
Gene symbol EPO/Erythropoietin
Gene ID (NCBI) 2056
RRIDAB_2918839
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

This antibody can be used to neutralize the bioactivity of EPO.