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Species Reactivity

Species Reactivity

Conjugate

Conjugate

NeutraKine® IFN-gamma Monoclonal antibody, PBS Only

NeutraKine® IFN-gamma Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

1E10G7

Cat no : 69007-1-PBS

Synonyms

Validation Data Gallery

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  • Neutralization experiment of NeutraKine® IFN-gamma using NeutraKine® IFN-gamma Monoclonal antibody (69007-1-Ig)

    Neutralization experiment of NeutraKine® IFN-gamma using 69007-1-Ig (same clone as 69007-1-PBS)

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (2ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. This data was developed using the same antibody clone with 69007-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IFN-gamma using NeutraKine® IFN-gamma Monoclonal antibody (69007-1-Ig)

    Neutralization experiment of NeutraKine® IFN-gamma using 69007-1-Ig (same clone as 69007-1-PBS)

    Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69007-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using NeutraKine® IFN-gamma Monoclonal antibody (69007-1-Ig)

    ELISA experiment of Recombinant protein using 69007-1-Ig (same clone as 69007-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69007-1-PBS in a different storage buffer formulation.

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Product Information

69007-1-PBS targets NeutraKine® IFN-gamma in Neutralization, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IFN-gamma protein HZ-1301 相同性解析による交差性が予測される生物種
Full Name IFN gamma
Gene symbol IFNG
Gene ID (NCBI) 3458
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.