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  • Neutralization experiment of NeutraKine® Noggin using NeutraKine® Noggin Mouse McAb (69010-1-Ig)

    Neutralization experiment of NeutraKine® Noggin using 69010-1-Ig (same clone as 69010-1-PBS)

    Recombinant human Noggin (Cat.NO. HZ-1118) inhibits BMP-4 induced alkaline phosphate production (using pNPP as chromogenic substrate for detection) by ATDC5 in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity of human Noggin (40 ng/mL HZ-1118) is neutralized by mouse anti-human Noggin monoclonal antibody 69010-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 5-20 μg/mL. The NeutraControl mouse anti-human Noggin monoclonal antibody 69510-1-Ig, generated from the same immunogen, could not neutralize human Noggin at the range of 0.0244-200 μg/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69010-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using NeutraKine® Noggin Mouse McAb (69010-1-Ig)

    ELISA experiment of Recombinant protein using 69010-1-Ig (same clone as 69010-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human Noggin (Cat.NO. HZ-1118) at 70 ng/well followed by blocking and adding serial diluted Noggin antibody 69010-1-Ig and 69510-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69010-1-PBS in a different storage buffer formulation.

NeutraKine® Noggin Monoclonal antibody, PBS Only

NeutraKine® Noggin Monoclonal Antibody for Neutralization, ELISA, Indirect ELISA
Cat No. 69010-1-PBS
Clone No.2A11C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA, Indirect ELISA

Synonyms

NOG, Noggin, 2A11C1, SYM1, SYNS1

Formulation:  Liquid
Lyophilized
Liquid
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Product Information

69010-1-PBS targets NeutraKine® Noggin in Neutralization, ELISA, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo:

Product name: HumanKine® recombinant human Noggin protein

Source: -derived,

Tag:

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name noggin
Gene Symbol Noggin
Gene ID (NCBI) 9241
Conjugate Unconjugated
Form
Purification MethodProtein G purification
UNIPROT IDQ13253
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

This antibody can be used to neutralize the bioactivity of Noggin.

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