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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse Neutrophil elastase Rabbit Recombinant (98197-1-RR)

    FC experiment of mouse bone marrow cells using 98197-1-RR

    1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse Neutrophil elastase Rabbit Recombinant (98197-1-RR)

    FC experiment of mouse bone marrow cells using 98197-1-RR

    1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98197-1-RR

    Affinity and Kinetic Characterization of 98197-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98197-1-RR against Mouse Neutrophil elastase were performed. The affinity constant is below 1 pM.

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Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody

Neutrophil elastase Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98197-1-RR
Clone No.241842G3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Leukocyte elastase, Elastase-2, Elane, Ela2, EC:3.4.21.37

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98197-1-RR targets Neutrophil elastase in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name elastase 2, neutrophil
Calculated molecular weight29KD
GenBank accession numberNM_015779.2
Gene Symbol Ela2
Gene ID (NCBI) 50701
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ3UP87
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for Neutrophil elastase antibody 98197-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols