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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nexilin Polyclonal antibody

Nexilin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27737-1-AP

Synonyms

F actin binding protein, NELIN, Nexilin, NEXN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nexilin Polyclonal antibody (27737-1-AP)

    WB analysis using 27737-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Nexilin Polyclonal antibody (27737-1-AP)

    IHC staining of mouse heart using 27737-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Nexilin Polyclonal antibody (27737-1-AP)

    IHC staining of mouse heart using 27737-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Nexilin Polyclonal antibody (27737-1-AP)

    IF Staining of mouse heart using 27737-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Nexilin Polyclonal antibody (27737-1-AP)

    IF Staining of mouse heart using 27737-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27737-1-AP targets Nexilin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nexilin fusion protein Ag26938 相同性解析による交差性が予測される生物種
Full Name nexilin (F actin binding protein)
Calculated molecular weight 675 aa, 81 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC114444
Gene symbol Nexilin
Gene ID (NCBI) 91624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).

Protocols

Product Specific Protocols
WB protocol for Nexilin antibody 27737-1-APDownload protocol
IHC protocol for Nexilin antibody 27737-1-APDownload protocol
IF protocol for Nexilin antibody 27737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols