Lipocalin-2/Ngal Recombinant antibody
Lipocalin-2/Ngal Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
230372D6
Cat no : 83102-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, mouse uterus tissue |
| Positive IHC detected in | mouse spleen tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83102-1-RR targets Lipocalin-2/Ngal in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | lipocalin 2 |
| Calculated molecular weight | 23KD |
| Observed molecular weight | 25 kDa |
| GenBank accession number | NM_008491.1 |
| Gene symbol | NGAL |
| Gene ID (NCBI) | 16819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Ngal.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Lipocalin-2/Ngal antibody 83102-1-RR | Download protocol |
| IHC protocol for Lipocalin-2/Ngal antibody 83102-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
