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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ninein Polyclonal antibody (13007-1-AP)

    WB analysis using 13007-1-AP (same clone as 13007-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13007-1-AP (Ninein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Polyclonal antibody (13007-1-AP)

    IHC staining of mouse brain using 13007-1-AP (same clone as 13007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Polyclonal antibody (13007-1-AP)

    IHC staining of mouse brain using 13007-1-AP (same clone as 13007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ninein Polyclonal antibody (13007-1-AP)

    IF Staining of HeLa using 13007-1-AP (same clone as 13007-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Ninein Polyclonal antibody (13007-1-AP)

    IF Staining of MCF-7 using 13007-1-AP (same clone as 13007-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Ninein Polyclonal antibody, PBS Only

Ninein Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13007-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13007-1-PBS targets Ninein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ninein fusion protein Ag3688 相同性解析による交差性が予測される生物種
Full Name ninein (GSK3B interacting protein)
Calculated molecular weight 2090 aa, 243 kDa
Observed molecular weight 240-255 kDa
GenBank accession numberBC034708
Gene Symbol Ninein
Gene ID (NCBI) 51199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4C6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.