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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ninein Monoclonal antibody, PBS Only

Ninein Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10A8

Cat no : 67132-1-PBS

Synonyms

GSK3B interacting protein, hNinein, KIAA1565, NIN, Ninein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ninein Monoclonal antibody (67132-1-Ig)

    WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Monoclonal antibody (67132-1-Ig)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Ninein Monoclonal antibody (67132-1-Ig)

    IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Ninein Monoclonal antibody (67132-1-Ig)

    IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Ninein Monoclonal antibody (67132-1-Ig)

    IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Ninein Monoclonal antibody (67132-1-Ig)

    IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67132-1-PBS targets Ninein in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Canine, mouse samples.

Tested Reactivity Human, Canine, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ninein fusion protein Ag14652 相同性解析による交差性が予測される生物種
Full Name ninein (GSK3B interacting protein)
Calculated molecular weight 2090 aa, 243 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC034708
Gene symbol NIN
Gene ID (NCBI) 51199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.