Nlrp3/NLRP3 Polyclonal antibody
Nlrp3/NLRP3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 30109-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | J774A.1 cells, rat spleen tissue, RAW 264.7 cells, LPS treated RAW 264.7 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
30109-1-AP targets Nlrp3/NLRP3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nlrp3/NLRP3 fusion protein Ag32515 相同性解析による交差性が予測される生物種 |
| Full Name | NLR family, pyrin domain containing 3 |
| Calculated molecular weight | 118kd |
| Observed molecular weight | 108-120 kDa |
| GenBank accession number | NM_145827 |
| Gene symbol | Nlrp3 |
| Gene ID (NCBI) | 216799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse Nlrp3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nlrp3/NLRP3 antibody 30109-1-AP | Download protocol |
| IF protocol for Nlrp3/NLRP3 antibody 30109-1-AP | Download protocol |
| IP protocol for Nlrp3/NLRP3 antibody 30109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JACC Basic Transl Sci PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia | ||
Sci Total Environ Intestinal microbiota via NLRP3 inflammasome dependent neuronal pyroptosis mediates anxiety-like behaviour in mice exposed to 3.5 GHz radiofrequency radiation | ||
Biochem Biophys Res Commun Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice | ||
Food Sci Biotechnol Geraniol alleviates liver injury induced by bisphenol A via modulating NLRP3/caspase-1 pathway and gut microbiota in mice model |
