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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUCB2/nesfatin-1 Polyclonal antibody

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26712-1-AP

Synonyms

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis using 26712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis of HEK-293T using 26712-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis using 26712-1-AP

    Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis of HepG2 using 26712-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis of mouse testis using 26712-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    WB analysis of rat brain using 26712-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    IF Staining of HepG2 using 26712-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (26712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)

    FC experiment of HepG2 using 26712-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, human milk, HEK-293T cells, rat brain tissue, HepG2 cells, mouse testis tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

26712-1-AP targets NUCB2/nesfatin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152 相同性解析による交差性が予測される生物種
Full Name nucleobindin 2
Calculated molecular weight 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_005013
Gene symbol NUCB2
Gene ID (NCBI) 4925
RRIDAB_2880610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Protocols

Product Specific Protocols
WB protocol for NUCB2/nesfatin-1 antibody 26712-1-APDownload protocol
IHC protocol for NUCB2/nesfatin-1 antibody 26712-1-APDownload protocol
IF protocol for NUCB2/nesfatin-1 antibody 26712-1-APDownload protocol
FC protocol for NUCB2/nesfatin-1 antibody 26712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

PMID: 29901268

Authors - Wang Li
View Article
humanWB

J Transl Med

NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway

Authors - Siyi Ning
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ARPE-19
NUCB2/nesfatin-1 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:26712-1-AP)