Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 26712-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 26712-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 26712-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 26712-1-AP
Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 26712-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 26712-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 26712-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (26712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of HepG2 using 26712-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NUCB2/nesfatin-1 Polyclonal antibody

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26712-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human milk, HEK-293T cells, rat brain tissue, HepG2 cells, mouse testis tissue
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 1 publications below

Product Information

26712-1-AP targets NUCB2/nesfatin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUCB2/nesfatin-1 fusion protein Ag25152 相同性解析による交差性が予測される生物種
Full Name	nucleobindin 2
Calculated molecular weight	50 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	NM_005013
Gene Symbol	NUCB2
Gene ID (NCBI)	4925
RRID	AB_2880610
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Protocols

Product Specific Protocols
WB protocol for NUCB2/nesfatin-1 antibody 26712-1-AP	Download protocol
IHC protocol for NUCB2/nesfatin-1 antibody 26712-1-AP	Download protocol
IF protocol for NUCB2/nesfatin-1 antibody 26712-1-AP	Download protocol
FC protocol for NUCB2/nesfatin-1 antibody 26712-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Proteomics PMID: 29901268 Authors - Wang Li View Article
human	WB	J Transl Med NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway Authors - Siyi Ning View Article
mouse,rat	WB,IF	Cell Commun Signal Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling Authors - Xue-Xue Zhu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26712-1-AP

WB analysis of HEK-293T using 26712-1-AP

WB analysis of rat brain using 26712-1-AP

WB analysis using 26712-1-AP

WB analysis of HepG2 using 26712-1-AP

WB analysis of mouse testis using 26712-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

IF Staining of HepG2 using 26712-1-AP

FC experiment of HepG2 using 26712-1-AP