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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    WB analysis of SH-SY5Y using 10975-2-AP (same clone as 10975-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    WB analysis of fetal human brain using 10975-2-AP (same clone as 10975-2-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Customer Sample customer using Nurr1/NR4A2 Polyclonal antibody (10975-2-AP)

    IF Staining of Customer Sample using 10975-2-AP (same clone as 10975-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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Nurr1/NR4A2 Polyclonal antibody, PBS Only

Nurr1/NR4A2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 10975-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

NURR1, NR4A2, Orphan nuclear receptor NURR1, NOT, Immediate-early response protein NOT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10975-2-PBS targets Nurr1/NR4A2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nurr1/NR4A2 fusion protein Ag1418 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 4, group A, member 2
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009288
Gene Symbol Nurr1
Gene ID (NCBI) 4929
RRIDAB_2153760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43354
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].