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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    WB analysis of HT-29 using 66878-1-Ig (same clone as 66878-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    WB analysis of Daudi using 66878-1-Ig (same clone as 66878-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    WB analysis of Ramos using 66878-1-Ig (same clone as 66878-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Nurr1/NR4A2 Monoclonal antibody (66878-1-Ig)

    IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Nurr1/NR4A2 Monoclonal antibody, PBS Only

Nurr1/NR4A2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66878-1-PBS
Clone No.1H5B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

NURR1, NR4A2, NOT, Immediate-early response protein NOT, HZF 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66878-1-PBS targets Nurr1/NR4A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nurr1/NR4A2 fusion protein Ag1418 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 4, group A, member 2
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC009288
Gene Symbol Nurr1
Gene ID (NCBI) 4929
RRIDAB_2882211
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43354
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].