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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using O-GlcNAc Monoclonal antibody (65292-1-Ig)

    WB analysis using 65292-1-Ig

    Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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O-GlcNAc Monoclonal antibody

O-GlcNAc Monoclonal Antibody for WB
Cat No. 65292-1-Ig
Clone No.CTD110.6

Host / Isotype

Mouse / IgM

Reactivity

n/a

Applications

WB

Synonyms

O-glycosylation, O-Glc, O Glc, glycosylation, O-linked β-N-acetylgulcosamine

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

For this antibody, it is recommended to use BSA as the blocking agent, avoiding using milk in the blocking or dilution buffers.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65292-1-Ig targets O-GlcNAc in WB applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name O-GlcNAc
Gene Symbol
Gene ID (NCBI)
RRIDAB_3670285
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).

Protocols

Product Specific Protocols
WB protocol for O-GlcNAc antibody 65292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols