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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OATL1 Polyclonal antibody

OATL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28597-1-AP

Synonyms

TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OATL1 Polyclonal antibody (28597-1-AP)

    WB analysis using 28597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using OATL1 Polyclonal antibody (28597-1-AP)

    WB analysis using 28597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28597-1-AP targets OATL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OATL1 fusion protein Ag29470 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 25
Observed molecular weight80-90 kDa
GenBank accession numberBC101817
Gene symbol TBC1D25
Gene ID (NCBI) 4943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OATL1, also known as TBC1D25, is a protein with a TBC domain and functions as a Rab GTPase activating protein. It is involved in the fusion of autophagosomes with endosomes and lysosomes. OATL1 is recruited to isolation membranes and autophagosomes through direct interaction with Atg8 homologues and is involved in the fusion between autophagosomes and lysosomes through its GAP activity.

Protocols

Product Specific Protocols
WB protocol for OATL1 antibody 28597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols