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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLCO1B3/OATP1B3 Polyclonal antibody (22815-1-AP)

    WB analysis using 22815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22815-1-AP (SLCO1B3/OATP1B3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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SLCO1B3/OATP1B3 Polyclonal antibody

SLCO1B3/OATP1B3 Polyclonal Antibody for WB, ELISA
Cat No. 22815-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, L02 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22815-1-AP targets SLCO1B3/OATP1B3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLCO1B3/OATP1B3 fusion protein Ag18940 相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 1B3
Calculated molecular weight 702 aa, 77 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC105597
Gene Symbol SLCO1B3
Gene ID (NCBI) 28234
RRIDAB_3669405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. SLCO1B3 is a glycosylated protein and can be detected in the non-glycosylated form at 70-75 kDa(PMID: 21278621).

Protocols

Product Specific Protocols
WB protocol for SLCO1B3/OATP1B3 antibody 22815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols