Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
Product Information
16719-1-AP targets OBFC2A in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OBFC2A fusion protein Ag10158 相同性解析による交差性が予測される生物種 |
| Full Name | oligonucleotide/oligosaccharide-binding fold containing 2A |
| Calculated molecular weight | 124 aa, 14 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC107723 |
| Gene Symbol | OBFC2A |
| Gene ID (NCBI) | 64859 |
| RRID | AB_2156021 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OBFC2A antibody 16719-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress. | ||
Cell Res The Integrator complex controls the termination of transcription at diverse classes of gene targets.
| ||
EMBO J The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo.
| ||
Front Oncol The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer. | ||
J Immunol SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.
| ||
Sci Rep hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage. |