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  • Featured Product
  • KD/KO Validated

OCIAD1 Monoclonal antibody, PBS Only (Capture)

OCIAD1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10C3

Cat no : 66698-1-PBS

Synonyms

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using OCIAD1 Monoclonal antibody (66698-1-Ig)

    WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    WB analysis of rat testis using 66698-1-Ig (same clone as 66698-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    WB analysis of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human thyroid cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human thyroid cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human kidney using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human kidney using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human pancreas cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of human pancreas cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IHC staining of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OCIAD1 Monoclonal antibody (66698-1-Ig)

    IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-2-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-3-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66698-1-PBS targets OCIAD1 as part of a matched antibody pair:

MP50614-1: 66698-1-PBS capture and 66698-2-PBS detection (validated in Cytometric bead array)

MP50614-2: 66698-1-PBS capture and 66698-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OCIAD1 fusion protein Ag9977 相同性解析による交差性が予測される生物種
Full Name OCIA domain containing 1
Calculated molecular weight 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC003409
Gene symbol OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2882051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).