Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW264.7 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23454-1-AP targets ODC1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ODC1 fusion protein Ag10927 相同性解析による交差性が予測される生物種 |
| Full Name | ornithine decarboxylase 1 |
| Calculated molecular weight | 461 aa, 51 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC025296 |
| Gene Symbol | ODC1 |
| Gene ID (NCBI) | 4953 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ODC1 antibody 23454-1-AP | Download protocol |
| IHC protocol for ODC1 antibody 23454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |