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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ODC1 Monoclonal antibody (67336-1-Ig)

    WB analysis of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

    WB result of ODC1 antibody (67336-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ODC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ODC1 Monoclonal antibody (67336-1-Ig)

    WB analysis of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ODC1 Monoclonal antibody (67336-1-Ig)

    WB analysis of human placenta using 67336-1-Ig (same clone as 67336-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using ODC1 Monoclonal antibody (67336-1-Ig)

    WB analysis of HUVEC using 67336-1-Ig (same clone as 67336-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ODC1 Monoclonal antibody (67336-1-Ig)

    IF Staining of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ODC1 antibody (67336-1-Ig, Clone: 2A1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ODC1 Monoclonal antibody, PBS Only

ODC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67336-1-PBS
Clone No.2A1E3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ODC, EC:4.1.1.17, 2A1E3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67336-1-PBS targets ODC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ODC1 fusion protein Ag10927 相同性解析による交差性が予測される生物種
Full Name ornithine decarboxylase 1
Calculated molecular weight 461 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC025296
Gene Symbol ODC1
Gene ID (NCBI) 4953
RRIDAB_2882594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11926
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.