Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
24736-1-AP targets ODF1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ODF1 fusion protein Ag20562 相同性解析による交差性が予測される生物種 |
| Full Name | outer dense fiber of sperm tails 1 |
| Calculated molecular weight | 250 aa, 28 kDa |
| Observed molecular weight | 28 kDa, 56 kDa |
| GenBank accession number | BC104457 |
| Gene Symbol | ODF1 |
| Gene ID (NCBI) | 4956 |
| RRID | AB_2879697 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ODF1, also named as Outer dense fiber protein 1, is a 250 amino acid protein, which contains the C-terminal contains many C-X-P repeats. ODF1 is a component of the outer dense fibers (ODF) of spermatozoa. ODF27 that suggested the possibility of inter- actions between ODF27 itself and/or between ODF27 and other sperm tail proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ODF1 antibody 24736-1-AP | Download protocol |
| IHC protocol for ODF1 antibody 24736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. | ||
Mol Genet Genomic Med Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects. | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice |