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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ODF3 Polyclonal antibody

ODF3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25097-1-AP

Synonyms

hSHIPPO, ODF3, Outer dense fiber protein 3, SHIPPO1, Sperm tail protein SHIPPO 1, TISP50

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat testis tissue using ODF3 Polyclonal antibody (25097-1-AP)

    WB analysis of rat testis using 25097-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 25097-1-AP (ODF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ODF3 Polyclonal antibody (25097-1-AP)

    IHC staining of human testis using 25097-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25097-1-AP (ODF3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ODF3 Polyclonal antibody (25097-1-AP)

    IHC staining of human testis using 25097-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25097-1-AP (ODF3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inrat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25097-1-AP is ODF3 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ODF3 fusion protein Ag18734 相同性解析による交差性が予測される生物種
Full Name outer dense fiber of sperm tails 3
Calculated molecular weight 254 aa, 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC126248
Gene symbol ODF3
Gene ID (NCBI) 113746
RRIDAB_2879894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ODF3 antibody 25097-1-APDownload protocol
IHC protocol for ODF3 antibody 25097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols