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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGDH Recombinant antibody, PBS Only

OGDH Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat no : 82773-1-PBS

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGDH Recombinant antibody (82773-1-RR)

    WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using OGDH Recombinant antibody (82773-1-RR)

    WB analysis of rat heart using 82773-1-RR (same clone as 82773-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82773-1-PBS targets OGDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OGDH fusion protein Ag7368 相同性解析による交差性が予測される生物種
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC004964
Gene symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.