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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGDH Recombinant antibody (82773-1-RR)

    WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using OGDH Recombinant antibody (82773-1-RR)

    WB analysis of rat heart using 82773-1-RR (same clone as 82773-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using OGDH Recombinant antibody (82773-1-RR)

    IHC staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using OGDH Recombinant antibody (82773-1-RR)

    IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using OGDH Recombinant antibody (82773-1-RR)

    IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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OGDH Recombinant antibody, PBS Only

OGDH Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 82773-1-PBS
Clone No.1C16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82773-1-PBS targets OGDH in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OGDH fusion protein Ag7368 相同性解析による交差性が予測される生物種
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.