- Featured Product
- KD/KO Validated
OGDHL Polyclonal antibody
OGDHL Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17110-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
17110-1-AP targets OGDHL in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OGDHL fusion protein Ag10794 相同性解析による交差性が予測される生物種 |
| Full Name | oxoglutarate dehydrogenase-like |
| Calculated molecular weight | 1010 aa, 115 kDa |
| Observed molecular weight | 115 kDa |
| GenBank accession number | BC026320 |
| Gene symbol | OGDHL |
| Gene ID (NCBI) | 55753 |
| RRID | AB_2156767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OGDHL antibody 17110-1-AP | Download protocol |
| IHC protocol for OGDHL antibody 17110-1-AP | Download protocol |
| IP protocol for OGDHL antibody 17110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
| ||
Nat Commun A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease | ||
Am J Hum Genet Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia. | ||
Front Cell Dev Biol Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism. | ||
Transl Psychiatry Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. | ||
Front Cardiovasc Med Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus. |
