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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGFOD2 Polyclonal antibody

OGFOD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21590-1-AP

Synonyms

OGFOD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGFOD2 Polyclonal antibody (21590-1-AP)

    WB analysis using 21590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21590-1-AP (OGFOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    WB analysis of human testis using 21590-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    WB analysis of human skeletal muscle using 21590-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    IHC staining of mouse brain using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    IHC staining of human placenta using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    IHC staining of human placenta using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using OGFOD2 Polyclonal antibody (21590-1-AP)

    IHC staining of mouse brain using 21590-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21590-1-AP (OGFOD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human testis tissue, human skeletal muscle tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21590-1-AP is OGFOD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGFOD2 fusion protein Ag16164 相同性解析による交差性が予測される生物種
Full Name 2-oxoglutarate and iron-dependent oxygenase domain containing 2
Calculated molecular weight 350 aa, 39 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC098293
Gene symbol OGFOD2
Gene ID (NCBI) 79676
RRIDAB_10734327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OGFOD2 belongs to the OGFOD2 family. OGFOD2 has 4 isoforms with the molecular mass of 15, 20, 32 and 39 kDa.

Protocols

Product Specific Protocols
WB protocol for OGFOD2 antibody 21590-1-APDownload protocol
IHC protocol for OGFOD2 antibody 21590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols