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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGG1 Polyclonal antibody

OGG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30302-1-AP

Synonyms

8 oxoguanine DNA glycosylase, AP lyase, HMMH, HOGG1, MMH, MUTM, OGG1, OGH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGG1 Polyclonal antibody (30302-1-AP)

    WB analysis using 30302-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30302-1-AP (OGG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30302-1-AP targets OGG1 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGG1 fusion protein Ag32962 相同性解析による交差性が予測される生物種
Full Name 8-oxoguanine DNA glycosylase
Calculated molecular weight 22 kDa, 36-40 kDa, 45-57 kDa
Observed molecular weight36-40 kDa
GenBank accession numberBC000657
Gene symbol OGG1
Gene ID (NCBI) 4968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).

Protocols

Product Specific Protocols
WB protocol for OGG1 antibody 30302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols