• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGN Monoclonal antibody

OGN Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5F11

Cat no : 66382-1-Ig

Synonyms

Mimecan, OG, OGN, OIF, osteoglycin, Osteoinductive factor, SLRR3A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human artery tissue using OGN Monoclonal antibody (66382-1-Ig)

    WB analysis of human artery using 66382-1-Ig

    Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig artery tissue using OGN Monoclonal antibody (66382-1-Ig)

    WB analysis of pig artery using 66382-1-Ig

    Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human lung using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using OGN Monoclonal antibody (66382-1-Ig)

    IHC staining of human lung using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inhuman artery tissue, pig artery tissue
Positive IHC detected inhuman liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66382-1-Ig targets OGN in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OGN fusion protein Ag3484 相同性解析による交差性が予測される生物種
Full Name osteoglycin
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC037273
Gene symbol OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Protocols

Product Specific Protocols
WB protocol for OGN antibody 66382-1-IgDownload protocol
IHC protocol for OGN antibody 66382-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols